Analytical Quality

From sample preparation to validated report — a look inside how we measure fatty acids with precision, consistency, and rigorous quality control.

Analytical Science

The Science Behind Your Results

Every result we report passes through a rigorous three-phase diagnostic process — from the moment your sample arrives to the final validated report.

Pre-Analytics
Analytics
Post-Analytics
Analytics = Fatty acid extraction → Fatty acid analysis — the core measurement step

Pre-analytics: why erythrocyte membranes

We measure fatty acids in red blood cell (erythrocyte) membranes — not plasma or serum. RBC membranes reflect your average fatty acid intake over the previous 90–120 days, much like HbA1c reflects blood glucose over time.

Long-term biomarker

Reflects 90–120 days of dietary intake, not a single meal. Gives a stable, meaningful clinical picture.

Low biological variability

RBC membrane composition is tightly regulated. Repeat measurements are consistent, making trends reliable.

Tissue-representative

Membrane fatty acids correlate strongly with levels in heart, brain, and other tissues — clinically relevant markers.

Sample collection: two paths, same result

Whether you collect via venous draw or finger-prick, our validated methods deliver equivalent results.

Venous blood (EDTA) Finger-prick (DBS)
Collection Phlebotomist draws into EDTA tube Self-collect finger-prick onto card
Precision Gold standard Equivalent after correction
Fasting Not required 10+ hours recommended — minimises plasma fatty acid interference from recent meals
Transport Ambient temperature, stable up to 7 days (14 days refrigerated) Room temperature post, stable 4+ weeks
Setting Clinical / pathology Home, clinic, field, remote
DBS–RBC equivalence: Dried blood spot measurements are converted to RBC-equivalent values using a validated correction factor. Published data show a correlation of r = 1.00 between the two methods, meaning DBS results are analytically interchangeable with venous RBC results.

Sample preparation workflow

Both sample types converge into an identical extraction process once prepared.

Venous blood
Centrifuge Separate layers
Remove plasma Isolate RBCs
Transfer To reaction vial
DBS
Punch out Dried blood spot
Transfer To reaction vial
Identical for both
Derivatise Chemical prep
Heat & mix Controlled reaction
Separate Centrifuge phases
Extract Into GC vial
Strict standardisation: Every variable in sample preparation is tightly controlled — reagent concentrations, reaction temperatures and times, solvent types, mixing intensity, light protection, vial types, and phase separation parameters. This ensures batch-to-batch reproducibility.

Analytics: gas chromatography (GC-FID)

Extracted fatty acids are analysed by gas chromatography with flame ionisation detection — the reference method for fatty acid quantification worldwide.

Identical instrument setup

Same GC model, column specifications (length, diameter, stationary phase), and detector configuration across all instruments.

Controlled gas supply

Constant flow rates of hydrogen, synthetic air, and nitrogen ensure reproducible retention times for every analyte.

Programmed oven cycles

Precise heating and cooling ramps separate fatty acids by chain length and saturation for accurate identification.

Post-analytics: validation before reporting

No result leaves the laboratory without passing through a multi-stage validation pipeline.

Visual data check
Automated evaluation
Technical validation
Lab director validation
Report

Chromatogram review

Every chromatogram and negative control is visually inspected for anomalies — split peaks, missing peaks, ghost peaks, or injection issues.

Automated range checks

Software automatically verifies that all measured fatty acids fall within established biological limits, flagging any suspicious or abnormal patterns.

Lab director sign-off

Flagged results undergo manual review by the lab director. Only after expert approval is a report issued — otherwise the sample is re-run.

Report or re-run

If any stage fails validation, the sample is repeated from scratch. We never release a result we can’t stand behind.

All checks pass → Report issued
Any flag → Sample re-run
Quality Assurance

Accuracy You Can Trust

Behind every report is a quality system designed to catch errors before they reach you. These are the safeguards built into every batch we run.

Control Samples in Every Batch

Known-value reference samples are processed alongside patient samples in every analytical run, confirming the system is performing within specification.

Documented Protocols

Every procedure follows a validated standard operating protocol. Methods are version-controlled and reviewed regularly to ensure consistency.

Instrument Maintenance

Scheduled calibration and preventive maintenance of all analytical instruments, with documented service records and performance verification.

Full Traceability

Every sample is tracked from receipt to report — reagent lot numbers, instrument logs, analyst identity, and processing timestamps are all recorded.

Validated Consumables

Reagents, columns, and consumables are verified against acceptance criteria before use. Out-of-spec materials are rejected and documented.

Deviation Management

Any unexpected result or process deviation triggers a formal investigation, root-cause analysis, and corrective action before work continues.

GC-FID Reference method
Every Batch Control samples
Full Audit Trail Sample to report

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